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Image Search Results
Journal: Molecular Cancer
Article Title: Gene expression time-series analysis of Camptothecin effects in U87-MG and DBTRG-05 glioblastoma cell lines
doi: 10.1186/1476-4598-7-66
Figure Lengend Snippet: Semi-quantitative RT-PCR post-validation of microarray results . A . Gene expression changes for seven genes (CDKN1A, GADD45, TXNIP, MYC, GEM, AREG, EREG) were measured at selected time points after CPT treatment by semi-quantitative RT-PCR and normalized to β-actin expression of time- and dose- matched controls. The RT-PCR reactions were analyzed with the DNA1000 kit of the Agilent 2100 Bioanalyzer to give a gel-like image. B . At each selected time point after CPT treatment, expression fold change values obtained with semi-quantitative RT-PCR and microarrays were compared.
Article Snippet: Slides were scanned in both Cy-3 and Cy-5 channels with
Techniques: Quantitative RT-PCR, Microarray, Expressing, Reverse Transcription Polymerase Chain Reaction